Effects of hypergravity on lung carcinoma cells maintained in continuous organotypic culture
Croute, F.; Gaubin, Y.; Prevost, M.C.; Beaupain, R.; Pianezzi, B.; Soleilhavoup, J.P.
Aviation Space and Environmental Medicine 61(11): 1002-1006
1990
ISSN/ISBN: 0095-6562 PMID: 2256871 Document Number: 363451
The effects of hypergravity levels ranging from 1 to 15 g were studied on A549 lung adenocarcinoma cell line, cultivated as nodules. This organotypic culture model preserves as closely as possible the cellular structures and differentiation functions of the in vivo situation. Nodules submitted to hypergravity conditions for 27 d did not show any change of cell growth, protein and DNA contents, compared with controls. Also, cellular differentiation, as regards intracellular phospholipid composition and more particularly phosphatidylcholine content, appeared undisturbed. The only obvious effect of hypergravity was a modification of the structural organization, with a disappearance of the large alveoli present at the surrounding of control nodules and the development of a dense cellular mass instead.