Semi-continuous microcarrier culture of rCHO cells secreting HBsAg by feeding microcarriers
Chen, Z.H.; Shi, Y.A.; Ding, J.C.
Chinese Journal of Biotechnology 7(2): 153-159
1991
ISSN/ISBN: 1042-749X PMID: 1806025 Document Number: 371520
On the basis of culturing rCHO cells semi-continuously with a constant concentration of microcarriers, cell yield and HBsAg expression were increased by feeding microcarriers, step by step, to provide a surface for cells to grow. A process of culturing rCHO cells semi-continuously to produce HBsAg by feeding microcarriers was established, in which the maximum microcarrier concentration was defined. The foundation has been laid for culturing rCHO cells continuously to reach high cell yield and high HBsAg expression.