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Constitutive production of a factor supporting B lymphocyte differentiation by a T cell hybridoma

Elkins, K.; Cambier, J.C.

Journal of Immunology 130(3): 1247-1251

1983

ISSN/ISBN: 0022-1767
PMID: 6337214
Document Number: 212180
To investigate the role of soluble T cell products during B cell differentiation more fully, T cell hybridomas were produced by the fusion of normal helper T cells with the T cell lymphoma BW5147. The production of one such hybrid, I4G3, the subclone I4G3.1F2 and the functional activity of the constitutive product are described. The hybrid supernatant acts exclusively in antigen-nonspecific, but antigen-dependent, promotion of B cell differentiation. It is optimally effective in the presence of small amounts of EL4 supernatant. It does not itself contain any detectable IL 2 [interleukin 2] or BCGF [B cell growth factor] or interferon activity. I4G3.1F2 activity is probably an important component of the conventional TRF [T cell replacing factor] preparations produced by mixed lymphocyte populations, and will be useful in further dissection of the contributions of different soluble T cell products to B cell differentiation.

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Carlsson, M.; Tötterman, T.H.; Rosén, A.; Nilsson, K. 1989: Interleukin-2 and a T cell hybridoma (MP6) derived B cell-stimulatory factor act synergistically to induce proliferation and differentiation of human B-chronic lymphocytic leukemia cells Leukemia 3(8): 593-601
Ohta, M.; Furukawa, Y.; Ide, C.; Akiyama, N.; Utakoji, T.; Miura, Y.; Saito, M. 1986: Establishment and characterization of four human monocytoid leukemia cell lines (JOSK-I, -S, -M and -K) with capabilities of monocyte-macrophage lineage differentiation and constitutive production of interleukin 1 Cancer Research 46(6): 3067-3074
Smith, K.A.; Gilbride, K.J.; Favata, M.F. 1980: Lymphocyte activating factor promotes T-cell growth factor production by cloned murine lymphoma cells Nature 287(5785): 853-855
Warrington, R.J. 1988: B cell differentiation factor production in systemic lupus erythematosus Journal of Rheumatology 15(1): 54-58
Landolfo, S.; Giovarelli, M.; Capusso, A.; Forni, G. 1979: Lymphokine production in primary mixed lymphocyte culture (MLC). III. Alloantigen signals cell-cell interactions involved in migration inhibition factor and immune interferon release Bollettino dell'Istituto Sieroterapico Milanese 58(2): 128-132
Harada, N.; Kikuchi, Y.; Tominaga, A.; Takaki, S.; Takatsu, K. 1985: BCGFIi activity on activated B cells of a purified murine T cell-replacing factor (TRF) from a T cell hybridoma (B151K12) Journal of Immunology 134(6): 3944-3951
Lee, J.C.; Hapel, A.J.; Ihle, J.N. 1982: Constitutive production of a unique lymphokine (IL 3) by the WEHI-3 cell line Journal of Immunology 128(6): 2393-2398
Sarmiento, M.; Loken, M.R.; Trowbridge, I.S.; Coffman, R.L.; Fitch, F.W. 1982: High molecular weight lymphocyte surface proteins are structurally related and are expressed on different cell populations at different times during lymphocyte maturation and differentiation Journal of Immunology 128(4): 1676-1684
Gillis, S.; Henney, C.S. 1981: The biochemical and biological characterization of lymphocyte regulatory molecules. VI. Generation of a B cell hybridoma whose antibody product inhibits interleukin 2 activity Journal of Immunology 126(5): 1978-1984
Huang, S.R. 1985: Preliminary report on the production of hybridoma cell lines secreting monoclonal antibodies against adult worm antigens of Paragonimus westermani Zhonghua Yi Xue Za Zhi 65(1): 41-43
Ozaki, S.; Durum, S.K.; Muegge, K.; York-Jolley, J.; Berzofsky, J.A. 1988: Production of T-T hybrids from T cell clones. Direct comparison between cloned T cells and T hybridoma cells derived from them Journal of Immunology 141(1): 71-78
Bazin, R.; Lemieux, R. 1987: Role of the macrophage-derived hybridoma growth factor in the in vitro and in vivo proliferation of newly formed B cell hybridomas Journal of Immunology 139(3): 780-787
Kitsukawa, K.; Nakamoto, A.; Saito, A. 1992: Correlation of tuberculin skin reaction with lymphocyte proliferation, interferon-gamma production and tumor necrosis factor-alpha production after in vitro stimulation with PPD and killed Mycobacterium tuberculosis using peripheral blood of healthy donors Kekkaku: 67(7): 521-528
Steff, A.M.; Bouaboula, M.; Mouchiroud, G.; Arnaud, S.; Roux, P.; Cassellas, P.; Piechaczyk, M. 1996: Isolation and characterization of c-fos-expressing murine bone marrow stromal cell lines supporting myeloid differentiation Leukemia 10(3): 505-513
Fulop, G.M.; Pietrangeli, C.E.; Osmond, D.G. 1986: Regulation of bone marrow lymphocyte production: IV. Altered kinetic steady state of lymphocyte production after chronic changes in exogenous stimuli Experimental Hematology 14(1): 27-34
Horton, J.E.; Oppenheim, J.J.; Mergenhagen, S.E.; Raisz, L.G. 1974: Macrophage-lymphocyte synergy in the production of osteoclast activating factor Journal of Immunology 113(4): 1278-1287
Shinohara, N.; Kern, M. 1976: Differentiation of lymphoid cells: B cell as a direct target and T cell as a regulator in lipopolysaccharide-enhanced induction of immunoglobulin production Journal of Immunology 116(6): 1607-1612
Mudde, G.C.; Verberne, C.J.; de Gast, G.C. 1984: Human tonsil B lymphocyte function. II. Pokeweed mitogen-induced plasma cell differentiation of B cell subpopulations expressing multiple heavy chain isotypes on their surface Journal of Immunology 133(4): 1896-1901
Rodriguez, M.A.; Ceuppens, J.L.; Goodwin, J.S. 1982: Regulation of IgM rheumatoid factor production in lymphocyte cultures from young and old subjects Journal of Immunology 128(6): 2422-2428
Velleman, S.G.; McFarland, D.C. 1999: Influence of fibroblast growth factor, insulin-like growth factor I, and transforming growth factor beta on satellite cell type i collagen expression and localization during differentiation Cytobios 100(394): 101-110