Resolution of valproic acid from deuterated analogues and their quantitation in plasma using capillary gas chromatography

Hoffman, D.J.; Porter, W.R.

Journal of Chromatography 276(2): 301-309

1983


ISSN/ISBN: 0021-9673
PMID: 6415082
Document Number: 210046
Quantitation of column coated with OV-351. A hexadeutero and 2 tetradeutero analogs of valproic acid had resolutions of at least 1.2 from valproic acid. Plasma samples were extracted with CCl4 following the addition of 2-ethylhexanoic acid as the internal standard. The method is sensitive to at least 0.5 .mu.g/ml and provides the capability of conducting absolute bioavailability and pulsed dosing studies with deuterated drug analog without a mass spectrometer.

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