Quantitation by gas chromatography-chemical ionization-mass spectrometry of phenylalanine mustard in plasma of patients
Pallante, S.L.; Fenselau, C.; Mennel, R.G.; Brundrett, R.B.; Appler, M.; Rosenshein, N.B.; Colvin, M.
Cancer Research 40(7): 2268-2272
1980
ISSN/ISBN: 0008-5472 PMID: 7388793 Document Number: 164274
An unambiguous and sensitive method based on gas chromatography-chemical ionization-mass spectrometry has been developed to quantitate L-phenylalanine mustard and has been applied to measure levels in plasma of five patients receiving 0.15 to 0.25 mg/kg (10 to 17 mg) of the drug p.o. Peak plasma levels of 50 to 190 ng/ml were found to occur between 0.7 and 2.3 hr after ingestion. The time for the plasma level to fall to one-half of the peak value varied from 0.6 to 3 hr, and very low levels (less than 2 ng/ml) were present by 24 hr.