Comparative characteristics of 2 morphometric methods of assessing the degree of liver fatty distrophy
Avilova, G.G.; Grodetskaia, N.S.; Dereviagin, V.A.; Tutnova, A.V.
Biulleten' Eksperimental'noi Biologii i Meditsiny 94(12): 122-124
1982
ISSN/ISBN: 0365-9615 PMID: 6758881 Document Number: 193197
The fat content in the liver of white male Wistar rats was measured over time (1, 2, 4, 8 and 14 days) after a single exposure to CCl4 within a wide concentration range (from 30 to 25700 mg/m3) by the two morphometric techniques: subjective-graduation and semi-automatic evaluation of the volumetric fat fraction, using a TAS unit (Leitz-Wetzlar). The results obtained with both the methods indicate that the CCl4 concentration equal to 260 mg/m3 is the threshold one as shown by the morphological signs of liver fatty dystrophy. The semi-automatic method offered enables an objective evaluation of the findings, making the research time shorter. To obtain adequate results with the method, it is necessary to observe the rules of the morphological material treatment (contrast selective color, uniform and sufficient thickness of the sections).