Enzymatic hydrolysis of cellulose. Regulatory effect of the non-soluble substrate on the effectiveness of the enzymatic reaction
Klesov, A.A.; Grigorash, S.Iu.
Biokhimiia 47(3): 409-418
1982
ISSN/ISBN: 0320-9725 PMID: 6803849 Document Number: 191261
One of the cellulase components, i.e., cellobiase, can be adsorbed on a cellulose surface with the concomitant decrease of activity (by 10 times or more). The specific activity of the adsorbed cellobiase depends on the enzyme concentration in the adsorption layer and is increased with the increase in the surface concentration of cellobiase. Variations in the amount of non-soluble cellulose and the corresponding changes in cellobiase activity in the system (as a result of the adsorption) can lead to a certain alteration in the shape of the kinetic curves for formation of intermediate cellobiose, which in its turn controls the rate of formation of the end product, i.e. glucose. Thus, the substrate surface causes a regulatory effect on the rate and kinetic mechanism of the enzymatic conversion of cellulose to glucose due to the adsorption effects. [Cellulase preparations from Trichoderma koningii, T. reesei, Aspergillus niger and Geotrichum candidum were used in this study.].