Determination of free and esterified cholesterol in human serum by high-performance liquid chromatography and optical activity detection
Kuo, J.C.; Yeung, E.S.
Journal of Chromatography 229(2): 293-300
1982
ISSN/ISBN: 0021-9673 PMID: 7096467 Document Number: 189948
A separation and detection scheme is presented for the determination of free, esterified and total cholesterol in human serum. Separation is accomplished by reversed-phase high-performance liquid chromatography and the eluate is monitored by the laser-based optical activity detector. The method is simple, accurate and has the advantage of specificity and selectivity when compared with the many methods commonly used.
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