Magnetic proton relaxation of aqueous solutions of ribonuclease in the temperature region of intramolecular melting
Sharimanov, I.G.; Grosesku, R.; Mrevlishvili, G.M.
Biofizika 27(5): 772-775
1982
ISSN/ISBN: 0006-3029 PMID: 7138922 Document Number: 189503
Thermal denaturation of ribonuclease at NMR frequencies 22, 75, and 90 MHz was studied by proton magnetic relaxation. It has been found that the pattern of proton relaxation in denaturated macromolecule is determined by slow exchange tau M approximately 10(-2)--10(-3) sec of "rigidly bound" water molecules and the solvent as a whole.