Ability of tumor cells to resist humoral vs. cell-mediated immune attack is controlled by different membrane physical properties
Schlager, S.I.
Biochemical and Biophysical Research Communications 106(1): 58-64
1982
ISSN/ISBN: 0006-291X PMID: 6809001 Document Number: 188447
Adriamycin-treated P815 tumor cells are increased in their killing by antibody plus complement (Ab-C) but not cytotoxic T lymphocytes (CTL). Mitomycin C-treated cells show increased killing by CTL but not by Ab-C. Hydrocortisone treatment decreases the killing of P815 cells by both Ab-C and CTL; epinephrine is ineffective in doing so. The drug and hormone effects are reversible. Adriamycin-treated P815 cells showed markedly increased plasma membrane fluidity. Mitomycin C-treated P815 cells showed markedly decreased net negative cell surface charge. Hydrocortisone-treated P815 cells showed decreased membrane fluidity and increased negative cell surface charge. There was no effect by mitomycin C on membrane fluidity, by adriamycin on surface charge, or by epinephrine on either membrane property. Reverted cells returned to control values of fluidity and cell surface charge. Plasma membrane fluidity and cell surface charge are apparently unique membrane physical properties that control tumor cell susceptibility to Ab-C and CTL killing, respectively.