Separation and direct quantitative estimation of adenine nucleotides on silufol
Zarubina, I.V.; Krivoruchko, B.I.
Ukrainskii Biokhimicheskii Zhurnal 54(4): 437-439
1982
ISSN/ISBN: 0201-8470 PMID: 6291206 Document Number: 185897
A chromatographic method is suggested for separating adenine nucleotides on Silufol UV-254 plates in a dioxane-isopropanol-ammonia-water (4:2:1:4) system and for their direct estimation in the thin layer by fluorimetric scanning of chromatograms. The method is applied in estimating adenine nucleotide content in the liver of intact rats and animals subjected to immobilization stress.