ATP, ADP and AMP content in the low-temperature preservation of lymphoid cells
Gordienko, A.D.; Fedets, O.I.
Voprosy Meditsinskoi Khimii 27(6): 746-749
1981
ISSN/ISBN: 0042-8809 PMID: 7336648 Document Number: 181812
As compared with ATP and ADP concentration in native lymphocytes, content of ATP was decreased down to 26.3% and 35.1% and of ADP--37.0% and 44.4% in the cell preparations after conservation in mixtures containing 10% PEO-400 and dimethyl-sulfoxide (DMSO). AMP was not found in the lymphocytes when both PEO-400 and DMSO were used. Mechanisms responsible for instability of lymphocyte adenine nucleotides under conditions of low temperature conservation are discussed.