Non-MHC-linked genetic control of murine cytotoxic T lymphocyte responses to hapten-modified syngeneic cells
Arora, P.K.; Shearer, G.M.
Journal of Immunology 127(5): 1822-1828
1981
ISSN/ISBN: 0022-1767 PMID: 6975299 Document Number: 181318
Spleen cells from unimmunized inbred mice were sensitized in vitro to autologous spleen cells modified with different concentrations of either trinitrobenzene sulfonate (TNBS) (TNP-self) or fluorescein isothiocyanate (FITC) (FTC-self). The resulting hapten-specific cytotoxic T lymphocyte (CTL) responses were assayed on hapten-self concanavalin A blast target cells. A number of mouse strains that expressed the H-2d, H-2k or H-2b haplotypes were studied to determine whether non-H-2-linked genes could affect CTL response potential. Among the 3 H-2d strains tested, BALB/c was a high responder, whereas B10.D2 and DBA/2 were low responders for CTL generated against TNP-self and FTC-self. Studies using (BALB/c .times. B10.D2)F1 responding cells and F1 or parental-modified stimulating cells indicated that the F1 cells generated cytotoxic activity equivalent to that of the high responder strain BALB/c. Among 3 H-2k strains studied, B10.BB mice were relatively low responders to TNP-self at all concentrations of TNBS stimulation. Among 4 H-2b strains compared, C57BL/6J, C3H.SW and A.BY were all observed to be relatively high responders, whereas C57BL/10J was the low responder strain for CTL responses to TNP-self. Further comparisons of the C57BL/6J and C57BL/10J strains indicated that these non-H-2-linked genes in the thymic environment.