Intercompartmental distribution of water after fluid infusion in dehydrated animal (II)
Takaori, M.; Kuroda, S.; Oosumi, A.
Masui. Japanese Journal of Anesthesiology 30(11): 1202-1208
1981
ISSN/ISBN: 0021-4892 PMID: 7338986 Document Number: 180036
Mongrel dogs (24) were restricted from water intake to induce a dehydrated state with water loss 6-7% of body weight within 2 days. They were anesthetized with pentobarbital and were ventilated mechanically. Six animals were left for 280 min under the control condition and were sacrificed by intentional ventricular fibrillation. The remaining 18 animals were infused with lactated Ringer's solution at 50 ml/kg per 60 min. They were divided into 3 groups of 6, and sacrificed at 90, 180 and 270 min after the infusion was started. All of the organs were frozen promptly after the ventricular fibrillation and were excised to be weighed. Total ECF volume, which was estimated by subtracting the organ ECF volume from the organ water content, remained unchanged for 90 min in all the organs, except in the brain. After 180 min, intracellular water influx was observed in the spleen, pancreas and skeletal muscle. Intracellular influx water was minimized in the stomach, small intestine and large intestine. The increases in ECF volume in those organs persisted even 270 min after the infusion. More rapid intracellular hydration estimated by measuring specific gravity was observed in the red cells.