Glucoamylase immobilization on silochrome using gossypol
El'chits, S.V.; Ivanova, L.A.; Khrobatenko, A.P.; Gernet, M.V.; Rakhimov, M.M.
Ukrainskii Biokhimicheskii Zhurnal 53(1): 21-25
1981
ISSN/ISBN: 0201-8470 PMID: 6259793 Document Number: 178922
The paper is concerned with conditions of glucoamylase binding with silanized silochrome using gossypol, dialdehyde isolated from cotton-plant. Kinetic properties of the immobilized enzyme are studied. The enzyme pH optimum does not change with immobilization and the temperature optimum is shifted from 50 degrees to 60 degrees C; a certain increase of the seeming Km is also observed. A high yield of the enzyme activity in immobilization evidences for the possibility of using gossypol as a binding agent in glucoamylase immobilization.