Studies on the male antifertility agent gossypol acetic acid. VII. Effect of motility stimulated factors on the revival of human spermatozoal motility after gossypol treatment in vitro
Kalla, N.R.; Gadru, N.; Foo, T.W.
Andrologia 18(4): 393-397
1986
ISSN/ISBN: 0303-4569 PMID: 3019180 DOI: 10.1111/j.1439-0272.1986.tb01797.xDocument Number: 353521
This brief experiment asked the question whether the inhibition of sperm motility imposed by gossypol is reversible by washing away the polyphenol. Human spermatozoa were incubated in Bakers buffer, 1 million/ml at 37 degrees C. for 30 minutes in the presence of 100 mc gossypol. Gossypol was dissolved in ethanol and diluted to 0.01% with distilled water, then 0.01 ml was added to 1.0 ml spermatozoa. Controls contained 0.1 ml of 0.01% ethyl acetate. Gossypol reduced motility from a range of 66.1 to 79.4% to a range of 32.1 to 38.1% (p0.01). Washing the spermatozoa by centrifuging twice at 343 x g for 10 min. and reincubating in a buffer had no effect on motility. Addition of the sperm motility stimulating agents theophylline 10 mM, kallikrein 100 KIU or dibutyryl-caMP after washing away the gossypol restored motility to 55.1%, 58.7% and 50.0%, respectively (p0.01).