Analysis of elements determining the generation of cytotoxic T lymphocytes. I. Irradiated F1 recipients in which antigenic stimulation occurs consistently at an excess dose
Nanishi, F.; Nomoto, K.; Taniguchi, K.; Kubo, C.; Takeya, K.
Japanese Journal of Experimental Medicine 51(1): 9-16
1981
ISSN/ISBN: 0021-5031 PMID: 6975842 Document Number: 176377
Specific cytotoxicity against histocompatibility antigens of a parental strain was induced in the spleens of sublethally irradiated Fl mice after i.v. lymphoid cell transfer from another parental strain. Such cytotoxicity reached the highest level at 10 days after the transfer of 3-5 .times. 107 spleen cells and was ascribed to T lymphocytes of donor parental origin. Secondary response of generation of toxicity was compared among in vivo rechallenge, in vitro MLC and transfer to irradiated F1 mice. Cytotoxicity was not so augmented by in vivo rechallenge. It may be attributable to early graft elimination. In in vitro MLC and transfer to irradiated F1 mice, cytotoxicity generation appeared earlier with higher magnitudes as compared to primary response. The transfer system to irradiated F1 recipients appears to be more suitable for analyses of cytotoxic T lymphocyte generation from memory cells than in vivo rechallenge.