Organ culture as a model for human pituitary tumour research
Anniko, M.
Laryngologie Rhinologie Otologie 60(6): 304-307
1981
ISSN/ISBN: 0340-1588 PMID: 7266196 Document Number: 175539
The organ culture technique based on material obtained from individual human pituitary tumors represents a model for the preservation of tissue in organotypic differentiation in which the tissue can be studied in the absence of vascular, hypothalamic and other neurogenic influences. In the organ culture system, 4 criteria are fulfilled: preserved morphology at the light microscopic and EM levels, preserved synthesis/secretion of those hormones as found in vivo, the same reactivity in vitro to physiological stimulators/inhibitors (TRH and dopamine) as in vivo and preservation of the same cell phase in the cell cycle and the same DNA content in cells in vitro as in vivo.