Culture of endothelial cells as an in vitro model for experimental and clinical research
Dzirlo-Todorović, J.
Medicinski Arhiv 50(3-4): 73-76
1996
ISSN/ISBN: 0025-8083 PMID: 9601757 Document Number: 466452
Culture of endothelial cell is gotten from human umbilical cord by enzymatic digestion. For the growth of cells in culture, medial RPMI 1640 with 20% mixed human serum (NHS) or 20% fetal calf serum (FCS), endothelial cell growth factor (ECGF) and heparin have been used. Plastic, 0.1% gelatin and fibronectin have been used as a fundament. Immune identification of endothelial cells was culture is performed by monoclonal antibodies on vWF:Ag. Homogeneous cell line in culture might be used as in vitro model in both experimental and practice work.