The protective effect of cholic acid on an in vitro injury model of ischemia-reperfusion in cerebral microvascular endothelial cells
Gao, Y-Hong.; Yuan, Z-Zhong.; Niu, F-Ling.; Cui, W.; Zhu, L-Qun.; Wang, S-Ren.
Zhongguo Zhong Yao Za Zhi 30(12): 930-932
2005
ISSN/ISBN: 1001-5302 PMID: 16124614 Document Number: 586465
Objective: To establish an in vitro injury model of ischemia-reperfusion in cerebral microvascular endothelial cells of rats and observe the protective effect of cholic acid. Method: Cultured rat microvascular endothelial cells were subjected to the oxygen-glucose deprivation (OGD) (Krebs solution) and recovery of oxygen-glucose, which simulated in vitro ischemia and reperfusion injury, and treated with cholic acid. The A value was measured with MU chromatometry. Result: Cultured cells were impaired after OGD for 4 hours and recovery of oxygen-glucose for 12 hours, the A value of the cells treated with cholic acid was significantly higher than that of the cells without treatment (P<0.01). Conclusion: Cholic acid could obviously protect rat cerebral microvascular endothelial cells from injury induced by an in vitro ischemia-reperfusion.