Protective effects of vitamin e on the vascular endothelial cells from oxidative injury by oxidized low-density lipoprotein in vitro
Chang, H.; Huang, G.; Liu, L.; Liu, Y.
Wei Sheng Yan Jiu 32(6): 576-578
2003
ISSN/ISBN: 1000-8020 PMID: 14963908 Document Number: 560087
To study the protective effect of vitamin E on vascular endothelial cells (VEC) damaged by oxidized low-density lipoprotein(ox-LDL). To establish the oxidative injury model of VEC induced by ox-LDL in vitro. The cells were divided into five groups(control group, ox-LDL group and VE-L + ox-LDL, VE-M + ox-LDL, VE-H + ox-LDL group). The cells were protected with varying concentrations of vitamin E solution for 24 hours before exposed to ox-LDL. After another 24 hours, the cells were collected and relative indexes were determined. The results showed that the level of MDA in ox-LDL group was higher than that in the other four groups(P < 0.05). The activities of SOD in ox-LDL group were lower than that in control group and three VE treated groups(P < 0.05). The activities of GSH-Px in ox-LDL group and VE-L + ox-LDL group were lower than that in other groups(P < 0.05). Vitamin E can prevent vascular endothelial cells from oxidized low-density lipoprotein induced oxidative injury.