Top Abstract Purchase Related
Home
>
Node
>
Subnode

An improved method for concurrent isolation of human granulocytes and lymphocytes. its application in isoelectric focusing electrophoresis

Poon, K.C.; Liu, P.I.; Chen, J.J.; Hong, C.C.; Allen, R.C.

Annals of Clinical and Laboratory Science 11(2): 115-118

1981

ISSN/ISBN: 0091-7370
PMID: 7259085
Document Number: 174491
A modified method of Boyum's technique to isolate granulocytes and lymphocytes from human blood is described. The granulocytes and the lymphocytes separated by this isolation procedure are highly pure, viable, and in sufficient number for subsequent isoelectric focusing studies. Different proteins have been separated by isoelectric focusing. This technique can be useful in the investigation of granulocytic and lymphocytic disorders.

Document emailed within 1 workday
Secure & encrypted payments

Leung, F.Y.; Henderson, A.R. 1981: Isolation and purification of aspartate aminotransferase isoenzymes from human liver by chromatography and isoelectric focusing Clinical Chemistry 27(2): 232-238
Rasulov, E.M.; Movsum-zade, K.M. 1985: Separation and identification of human globin chains by the method of isoelectric focusing Laboratornoe Delo 9: 527-530
Johnson, A.M. 1978: Immunofixation following electrophoresis or isoelectric focusing for identification and phenotyping of proteins Annals of Clinical and Laboratory Science 8(3): 195-200
Derkach, I.S.; Uryvaev, L.V.; Zhdanov, V.M. 1972: Study of protein components of the Venezuelan equine encephalomyelitis virus by electrophoresis and isoelectric focusing Voprosy Virusologii 17(2): 211-214
Rani, S.; Dhar, S.; Beohar, P.C.; Rao, K.V. 1982: Isoelectric focusing--a new marker for differentiating T & B lymphocytes Indian Journal of Experimental Biology 20(2): 172-174
Shan, D.E.; Yeh, S.I. 1998: Detection of oligoclonal bands in patients with neurologic diseases: comparison between agarose gel-, immunofixation- and isoelectric-focusing electrophoresis Zhonghua Yi Xue Za Zhi 61(7): 375-381
Tsibul'skaia, L.M.; Levina, A.A.; Andreeva, A.P.; Tokarev, I.N. 1977: Method of isoelectric focusing for detection of hemoglobinopathies Problemy Gematologii i Perelivaniia Krovi 22(4): 56-58
Liu, X.; Wang, Q.; Ma, L. 1997: Capillary isoelectric focusing method and its applications Se Pu 15(5): 400-404
Sutton, J.G. 1979: Further alleles of phosphoglucomutase in human semen detected by isoelectric focusing Journal of Forensic Sciences 24(1): 189-192
Wetzel, V.; Schaumlöffel, E. 1976: Isoelectric focusing of an alkylating 3H-cyclophosphamide derivative from human bladder urine International Journal of Clinical Pharmacology and Biopharmacy 14(4): 279-283
Pellikka, P.A.; Sullivan, W.P.; Coulam, C.B.; Toft, D.O. 1983: Comparison of estrogen receptors in human premenopausal and postmenopausal uteri using isoelectric focusing Obstetrics and Gynecology 62(4): 430-434
Thomas, J.W.; Virta, V.J.; Nell, L.J. 1985: Heterogeneity and specificity of human anti-insulin antibodies determined by isoelectric focusing Journal of Immunology 134(2): 1048-1052
Gmajnicki, B.; Kalenić, S. 1986: An improved method for isolation of salmonellae from human feces Lijecnicki Vjesnik 108(10): 431-433
Gustafsson, J.A.; Gustafsson, S.A.; Nordenskjöld, B.; Okret, S.; Silfverswärd, C.; Wrange, O. 1978: Estradiol receptor analysis in human breast cancer tissue by isoelectric focusing in polyacrylamide gel Cancer Research 38(11 Part 2): 4225-4228
Evelo, C.T.; Niessen, H.J.; Roelofs, H.M.; Henderson, P.T. 1987: Cysteine adducts of human haemoglobin measured by isoelectric focusing in polyacrylamide gels with a non-linear pH gradient Journal of Chromatography 420(1): 35-42
Arita, S.; Smith, C.V.; Nagai, T.; Sakamoto, Y.; Ochiai, M.; Maruyama, M.; Tanabe, Y.; Shelvin, L.; Mullen, Y. 1999: Improved human islet isolation by a tube method for collagenase infusion Transplantation 68(5): 705-707
Gusev, A.I.; Iazova, A.K. 1970: Isolation and purification of human and animal embryo specific alpha-globulins by the method of preparatory disk electrophoresis in polyacrylamide gel Biokhimiia 35(1): 172-181
Raghunathan, R.; Wuest, C.; Faust, J.; Hwang, S.; Miller, M.E. 1982: Isolation of ovine lymphocytes, granulocytes, and monocytes by counterflow centrifugation elutriation American Journal of Veterinary Research 43(8): 1467-1470
Viviani, G.L.; Bottino, R.; Zecchi, A.; Sanna, R.; Cavo, M.; Fontana, I.; Arcuri, V.; Lione, L.; Stubinski, R.; Falzetti, G. 1994: Application of the hydraulic shaking system method to isolation of human pancreatic islets Transplantation Proceedings 26(6): 3436-3437
Stepanova, T.N.; Iulikova, E.P.; Maksimova, R.A.; Silaeva, A.B.; Andreenko, G.V. 1977: Fractionation of "tricholysine" by isoelectric focusing Biokhimiia 42(1): 167-170