Effect of mitogens on suppression of antibody formation and proliferation in dense cultures
Korukova, A.A.; Kim, A.B.; Gurvich, A.E.
Biulleten' Eksperimental'noi Biologii i Meditsiny 91(2): 188-190
1981
ISSN/ISBN: 0365-9615 PMID: 6164414 Document Number: 173466
A study was made of the effect of mitogens on general proliferation and primary immune response to sheep red blood cells in density-inhibited cultures of mouse spleen cells. The mitogens applied included fetal calf serum and both B cell- and T cell-specific mitogens (dextran sulfate, LPS and ConA). Experiments with 3H-thymidine incorporation demonstrated that the proliferation was equally enhanced by any mitogen in both optimal and density-inhibited cultures. The mitogens did not remove the density inhibition of antibody formation.