Formation of IgE-binding factors by rat T lymphocytes. I. Induction of IgE-binding factors by poly I:C and interferon
Yodoi, J.; Hirashima, M.; Bloom, B.R.; Ishizaka, K.
Journal of Immunology 127(4): 1579-1585
1981
ISSN/ISBN: 0022-1767 PMID: 6974199 Document Number: 170609
Rat serum obtained 8-18 h after an i.p. injection of poly(I:C) contained IgE-binding factors, which can be detected by the inhibition of rosette formation of IgE Fc receptor-positive [Fc.epsilon.R(+)] cells with IgE-coated erythrocytes. Incubation of normal rat spleen cells with 1 .mu.g/ml poly(I:C) resulted in the formation of IgE-binding factors. Approximately 1/2 of the IgE-binding factors in the culture supernatant were IgE-potentiating factors which have affinity for concanavalin A (Con A); the remaining half of the factors were IgE-suppressive factors with no affinity for the lectin. Culture supernatants of poly(I:C)-stimulated spleen cells and the serum of poly(I:C) treated rats contained not only IgE-binding factors, but also soluble factors that induce normal lymphocytes to form IgE-binding factors. Poly(I:C)-stimulated splenic adherent cells and peritoneal macrophages formed soluble factors, which in turn induced T lymphocytes to express Fc.epsilon.R and to form IgE-binding factors. The inducers derived from poly(I:C)-stimulated cells failed to augment the expression of Fc.epsilon.R on B cells. The soluble factors could induce the formation of both IgE-potentiating factors and IgE-suppressive factors. Upon incubation with the inducers, 10 .mu.g/ml Con A-activated cells formed IgE-potentiating factors; 1 .mu.g/ml Con A-activated cells formed IgE-suppressive factors. The inducers had affinity for poly(U) and blue dextran Sepharose and could be recovered from the beads by elution with 1 M NaCl. Supernatant of mouse spleen cells co-cultured with measles virus-infected human cervical carcinoma Hela cells induced normal rat lymphocytes to form IgE-binding factors. The inducers in the culture supernatants were neutralized by a minute dose of antibodies specific for mouse type 1 interferon, indicating that mouse interferon can induce the formation of IgE-binding factors.