Regions of the CD4 molecule not involved in virus binding or syncytia formation are required for HIV-1 infection of lymphocytes
Hasunuma, T.; Tsubota, H.; Watanabe, M.; Chen, Z.W.; Lord, C.I.; Burkly, L.C.; Daley, J.F.; Letvin, N.L.
Journal of Immunology 148(6): 1841-1846
1992
ISSN/ISBN: 0022-1767 PMID: 1371792 Document Number: 394293
Cell surface-expressed CD4 binds to the envelope glycoprotein of HIV-1 and mediates syncytia formation through interacting with membrane expressed HIV-1 gp120. Further possible roles of the CD4 molecule in the process of cell infection by HIV-1 remain poorly understood. In our study we describe two mAb that recognize the V3/V4 domain of the CD4 molecule. Although these mAb do not inhibit gp120-CD4 binding or HIV-1-induced syncytia formation, they inhibit HIV-1 infection of human PBL. These findings suggest that discrete, definable domains of the CD4 molecule may be involved in interactions after HIV-1 envelope binding that lead to virus entry into the cell.