Properties of alpha-chymotrypsin, covalently incorporated into polyacrylamide microgranules
Iukasheva, E.V.; Aisina, R.B.; Kazanska, R.B.; Kazanskaia, N.F.; Eremeev, N.L.
Biokhimiia 45(3): 449-454
1980
ISSN/ISBN: 0320-9725 PMID: 7378483 Document Number: 168262
Polyacrylamide beads (microgranules) containing covalently bound alpha-chymotrypsin were obtained. An average diameter of the microgranules is 100--150 micrometers. Prior to the bead polymerization alpha-chymotrypsin was modified by acryloyl chloride. The efficiency of the microgranulated alpha-chymotrypsin action depends on the kinetic properties of the substrates used. The pH optimum for the microgranulated enzyme activity towards N-acetyl-L-tyrosine ethyl ester is shifted by 1,5 units to the alkaline zone. The immobilized alpha-chymotrypsin is completely stable for 100 hrs at 50 degrees.