Fluorometric DNA assay at nanogram levels in biological material
Cesarone, C.F.; Bolognesi, C.; Santi, L.
Bollettino della Societa Italiana di Biologia Sperimentale 56(16): 1666-1672
1980
ISSN/ISBN: 0037-8771 PMID: 7459087 Document Number: 163823
A high degree of reproducibility was obtained using a simple and rapid microfluorimetric method of DNA determination. The described method evaluates the increased fluorescence after reaction of 33258 Hoechst fluorochrome with calf thymus DNA and mouse liver chromatin preparations. The routinely used preparation reagents and the subcellular components do not interfere with the reaction.
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