A quantitative fluorometric assay for detection and characterization of Fc receptors
Schreiber, A.B.; Hoebeke, J.; Bergman, Y.; Haimovich, J.; Strosberg, A.D.
Journal of Immunology 121(1): 19-23
1978
ISSN/ISBN: 0022-1767 PMID: 670698 Document Number: 135776
A new quantitative fluorometric binding assay that uses fluoresceinated aggregated Ig cell lines (38C-13, EL4 and BW). The apparent association constant of the binding and the amount of aggregated IgG bound per cell at saturation were calculated. The fluorometric assay enables the detection of 5 .times. 10-10 M bound aggregated IgG. Inhibition studies with monomeric IgG, reduced and alkylated aggregated IgG and aggregated F(ab')2 fragments of IgG confirmed the specificity of the assay. Staphylococcal protein A inhibited the binding of the aggregated IgG to Fc receptors.