Lipopolysaccharide-induced immunomodulation of the generation cell-mediated cytotoxicity. II. Evidence for the involvement of a regulatory B lymphocyte
Vallera, D.A.; Gamble, C.E.; Schmidtke, J.R.
Journal of Immunology 124(2): 641-649
1980
ISSN/ISBN: 0022-1767 PMID: 6444315 Document Number: 163092
Bacterial enriched) (B-NWA) fraction was susceptible to the effects of LPS. When T-NWNA and B-NWA cells were mixed and used as responders in the MLC, LPS exerted its reductive effect in the presence of B-NWA cells. Proliferation of the B-NWA cells was a prerequisite for LPS-induced suppression of CMC since treatment of these cells with mitomycin C totally abrogated LPS-induced suppression of CMC. Involvement of B lymphocytes was further supported by the inability to demonstrate LPS induced suppression of CMC when C3H/HeJ cells were used as responders. The involvement of macrophages in this system was minimized. Despite separation of the BALB/c B-NWA cells from BALB/c T-NWNA cells and X-rayed C57BL/6 stimulator cells by an impermeable nuclepore membrane, LPS-induced suppression of CMC still occurred. LPS apparently induced a factor from activated B cells that had a pronounced modulatory effect on the T cell subpopulation(s) involved in the generation of CMC in the murine MLC.