Factors increasing the effectiveness of Bacillus subtilis fusion
Arbuzov, V.N.; Kondrat'ev, I.S.; Skavronskaia, A.G.
Biulleten' Eksperimental'noi Biologii i Meditsiny 89(5): 601-603
1980
ISSN/ISBN: 0365-9615 PMID: 6772256 Document Number: 160895
The factors potentiating the efficacy of fusion of protoplasts of Bacillus subtilis were studied. A 50-fold increase in the yield of primary prototrophs was provided by 90-minute preincubation of protoplasts in a liquid hyperbaric medium and 10-minute treatment with trypsin. Increased efficacy of fusion of protoplasts depended on their regeneration percentage.