Effect of postmortem autolysis on the 40S ribosomal subunit proteins of rat skeletal muscles
Shishkin, S.S.; Kitavtsev, B.A.; Dabov, S.S.
Voprosy Meditsinskoi Khimii 26(4): 476-480
1980
ISSN/ISBN: 0042-8809 PMID: 7456386 Document Number: 160221
A ribosomal protein component was impaired during autolysis of 40S subunits, obtained from polysomes of autolysed muscles using KCL-puromycine method. These 40S subunits exhibited higher buoyant density as compared with controls. For the direct analysis the ribosomal proteins were extracted from 40S subunits by 66% acetic acid containing 0.01 M MgCl2 and fractionated using one- and two-dimensional polyacrylamide gel electrophoresis. The data obtained suggest that some proteins were hydrolyzed by proteolytic enzymes during autolysis: assumption of dissociation into the subunits of 6 of the ribosomal proteins during autolysis does not account for the phenomena observed.