Effect of the 5-hydroperoxide of eicosatetraenoic acid and inhibitors of the lipoxygenase pathway on the formation of slow reacting substance by rat basophilic leukemia cells; direct evidence that slow reacting substance is a product of the lipoxygenase pathway

Falkenhein, S.F.; Macdonald, H.; Huber, M.M.; Koch, D.; Parker, C.W.; Falkenhein, S.F; Macdonald, H; Huber, M.M; Koch, D; Parker, C.W

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Effect of the 5-hydroperoxide of eicosatetraenoic acid and inhibitors of the lipoxygenase pathway on the formation of slow reacting substance by rat basophilic leukemia cells; direct evidence that slow reacting substance is a product of the lipoxygenase pathway

Falkenhein, S.F.; Macdonald, H.; Huber, M.M.; Koch, D.; Parker, C.W.

Journal of Immunology 125(1): 163-168

1980

ISSN/ISBN: 0022-1767

PMID: 6103910

Document Number: 158542

Previous studies in a line of rat basophilic leukemia (RBL-1) cells indicated that the slow reacting substance (SRS) made during stimulation with the divalent cation ionophore A23187 is derived from arachidonic acid (AA). Various inhibitors of AA metabolism were compared with regard to their effects on SRS formation and incorporation of radioactivity from [1-14C]-AA into known metabolites of the lipoxygenase and cyclooxygenase pathways. An apparently close parallel between lipoxygenase product formation and SRS synthesis is demonstrated. Exogenous 5-hydroperoxy-eicosatetraenoic acid markedly enhanced SRS synthesis even when A23187 was absent. SRS is apparently produced through the lipoxygenase pathway.