Thymocyte cyclic AMP and cyclic GMP response to treatment with metabolites issued from the lipoxygenase pathway
Mexmain, S.; Cook, J.; Aldigier, J.C.; Gualde, N.; Rigaud, M.
Journal of Immunology 135(2): 1361-1365
1985
ISSN/ISBN: 0022-1767 PMID: 2989363 Document Number: 260957
Evidence has been presented that cGMP is the 2nd messenger for the lipoxygenase metabolites 15-HETE (15-hydroxyperoxyeicosatetraenoic acid) and LTB4 (leukotriene B4) in the mouse splenocyte and thymocyte. Incubation of splenocytes with 10-7-10-9 M 15-HETE caused a slight decrease in cAMP levels and an increase in cGMP levels after 10-20 min. Mature PNA- (peanut agglutinin-negative), immature PNA+, and whole thymocytes treated with 107-1010 M 15-HETE and 10-11 M LTB4 showed an .apprx. 100% increase in cGMP production. In mixed lymphocyte reactions, 15-HETE- and LTB4-treated PNA+, PNA-, and whole thymocyte populations inhibited thymidine uptake by fresh allostimulated splenocytes. These results demonstrate that the eicosanoid-induced generation of suppressor cells follows a rise in lymphocyte cGMP levels.