A system for quality control of biotransformation enzymes in the mucosa of small intestine
Heimsch, E.; Dobler, T.
Journal of Clinical Chemistry and Clinical Biochemistry 18(11): 775-780
1980
ISSN/ISBN: 0340-076X PMID: 6777452 Document Number: 157796
A system for quality control of the determination of arylhydrocarbonhydroxylase (benzpyrenhydroxylase), ethoxycoumarin-O-deethylase, NADPH-cytochrom-c-reductase, epoxidehydrase and uridindiphosphoglucouronyltransferase was developed. We used lyophilized and frozen samples of postmitochondrial supernatant of rat and guinea pig small intestinal mucosa, which was prepared using a 1:5 ratio of mucosa to K-phosphate buffer. For NADPH-cytochrome-c-reductase, the microsomal suspension from rat small intestine was used. The samples were stored at -30 degrees C. The coefficient of variation within run was about 5% (15 samples), the coefficient of variation between run (15 days) about 10%. The control specimens were stable for 6 months.