Separation of human B lymphocytes by combined rosetting with mouse and sheep red cells
Matej, H.; Kunicka, J.
Archivum Immunologiae et Therapiae Experimentalis 28(1): 45-51
1980
ISSN/ISBN: 0004-069X PMID: 6968193 Document Number: 157439
A technique for obtaining a highly enriched B lymphocyte subpoplation from isolated peripheral blood lymphocytes is described. The method consists in combined use of mouse and sheep red cell rosettes. In the 1st step of the method, B cells-containing fraction of lymphocytes and papainized mouse red cells form rosettes which are separated by Ficoll-Hypaque gradient centrifugation. In the 2nd step, after lysis of mouse erythrocytes in the sediment, the recovered B cell rich fraction is purified by E rosetting procedures. B lymphocyte subpopulation obtained in this way presents a good viability and is almost completely pure.