Response of human B cells to Staphylococcus aureus Cowan I: T-independent proliferation and T-dependent differentiation to immunoglobulin secretion involve subsets separable by rosetting with mouse erythrocytes
Ito, S.; Lawton, A.R.
Journal of Immunology 133(4): 1891-1895
1984
ISSN/ISBN: 0022-1767 PMID: 6332138 Document Number: 221446
T-depleted mononuclear cells were separated into subsets by rosetting with mouse erythrocytes. Proliferation and differentiation responses to S. aureus Cowan I (SAC), pokeweed mitogen (PWM) and a combination of the 2 polyclonal activators were studied. All of the T cell-dependent proliferation of unfractionated B cells in response to SAC was attributable to mouse erythrocyte rosette-forming cells (BMR+). BMR- cells were not stimulated to proliferate by SAC in the presence or absence of T cells, but did proliferate to PWM plus irradiated T cells. Co-stimulation of BMR+ cells with SAC and PWM in the presence of autologous T cells did not lead to Ig secretion. The B cells stimulated to divide by SAC apparently do not become response to B cell differentiation factors and are distinct from those that undergo T cell-dependent differentiation.