In vitro cultivation of microfilaria of Dirofilaria immitis

Ramanna, B.C.; Singh, T.; Rao, C.K.; Rao, C.K.

Journal of Communicable Diseases 12(2): 95-97

1980


ISSN/ISBN: 0019-5138
PMID: 7310103
Document Number: 157122
Microfilariae of Dirofilaria immitis were cultivated in various media at 24 deg C. In RPMI-1640 tissue culture medium plus 10% bovine foetal serum, 55% of the larvae survived for 21 days and 15% developed to the sausage stage by the 6th day.

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