Investigations into the binding of phenprocoumon to albumin using fluorescence spectroscopy
Otagiri, M.; Fleitman, J.S.; Perrin, J.H.
Journal of Pharmacy and Pharmacology 32(7): 478-482
1980
ISSN/ISBN: 0022-3573 PMID: 6105183 Document Number: 155027
The fluorescence of phenprocoumon is enhanced following interaction with bovine and human serum albumin, allowing the binding parameters for 2 binding sites to be estimated by a modified Bjerrum method and the Scatchard method. The fluorescence is incresed as the pH is raised to 9.0 and on addition of the drugs ibuprofen and fenoprofen. Chloride ions, phenylbutazone and acenocoumarin seem to displace phenprocoumon from its primary binding site on human serum albumin. Investigations with the enantiomers of phenprocoumon show that the binding sites have some stereospecificity.