Macrophage-independent activation of helper T cells. I. Production of Interleukin 2
Farrar, J.J.; Mizel, S.B.; Fuller-Farrar, J.; Farrar, W.L.; Hilfiker, M.L.
Journal of Immunology 125(2): 793-798
1980
ISSN/ISBN: 0022-1767 PMID: 6967089 Document Number: 154894
Interleukin 2 is an antigen nonspecific soluble factor produced by concanavalin macrophage cell line culture supernatant or phorbol myristic acetate (4-20 ng/ml). Splenic macrophages stimulated with Con A and phorbol myristic acetate did not produce detectable IL 2. Gel filtration of a culture supernatant from purified T lymphocytes stimulated with Con A and phorbol myristic acetate revealed that the IL 2 had the MW and additional biologic properties (helper activity for antibody synthesis and T lymphocyte growth promoting activity) characteristic of Interleukin 2 produced by unfractionated Con A-stimulated spleen cells.