Effect of heat and selected chemical disinfectants upon infectivity of spores of Bacillus piliformis (Tyzzer's disease)
Ganaway, J.R.
Laboratory Animal Science 30(2 Pt 1): 192-196
1980
ISSN/ISBN: 0023-6764 PMID: 7052374 Document Number: 154486
Spores were not affected by heat treatment at 60 deg C, but were rendered uninfective by treatment at 70 and 80 deg C. Infectivity was not restored by treatment with trypsin. Infectivity was not lost when spores were treated with a phenolic germicidal detergent, ethanol, or one of two quaternary ammonium compounds containing 9% or 17% benzalkonium chloride. A graded effect was observed with formaldehyde solution and an iodophor. Spores were rendered uninfective by treatment with peracetic acid (1%) and a wetting agent, sodium alkylarylsulfonate or sodium hypochlorite solution (0.3%) for 5 minutes; infectivity was not restored by trypsin treatment. The probable means of transmission of Bacillus piliformis was discussed. Sodium hypochlorite solution (0.3%) was recommended as a surface disinfectant for animal facilities contaminated with the Tyzzer's disease agent.