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Factors affecting the course of DNA acid hydrolysis in carrying out the Feulgen reaction

Kotel'nikov, V.M.; Litinskaia, L.L.

Tsitologiia 21(5): 491-507

1979

ISSN/ISBN: 0041-3771
PMID: 380085
Document Number: 150072
Literature data concerning acid hydrolysis of DNA during the Feulgen procedure are reviewed, with emphasis being made on the dependence of Schiff-apurinic acid binding on the fixation technique, the temperature of hydrolysis and acid concentration, the rate of extraction of depolymerized DNA fragments, the nucleotide composition of DNA, the chromatin state, and on the composition of nucleoprotein. Some practical considerations for optimization of the Feulgen procedure for a precise quantitative determination of DNA amount are given.

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