An ELISA technic for the diagnosis of human African trypanosomiasis
Picq, J.J.; Faugère, B.; Sebeo, D.; Poncy, J.M.
Bulletin de la Societe de Pathologie Exotique et de Ses Filiales 72(3): 231-237
1979
ISSN/ISBN: 0037-9085 PMID: 400123 Document Number: 148598
The authors studied several factor influencing the serodiagnosis of human african trypanosomiasis using the microplate enzyme immunoassay ("micro E.L.I.S.A."). They used an antigenic extract obtained by sonic disruption of purified suspensions of trypanosomes of a Trypanosoma brucei brucei strain. the conjugate was a peroxidase-conjugate anti-human immunoglobulin and the chromogen was orthodianisidine. Spectrophotometric reading was used. Although this method may have some advantaged, its specificity needs further study because of few false positive and negative reactions. Moreover its reproducibility as quantitative reaction has proved to be delicate.