Localization of adenine components in chick myofibrils by the ferritin antibody technic
Ogievetskaia, M.M.; Podgorsdnichenko, V.K.; Samosudova, N.V.
Biofizika 24(1): 101-103
1979
ISSN/ISBN: 0006-3029 PMID: 435520 Document Number: 147413
The immunochemical method has been proposed to indicate the adenin compounds in isolated myofibrils of striated muscle. Adenosine--containing antigen was prepared by coupling adenosine with bovine serum albumin (adenosine--BSA). Antibodies against adenosine--BSA were labelled by ferritin. The sites of the antigen antibody reaction were marked by localization of ferritin molecules. Ferritin antibodies were found to concentrate in the following sarcomere sites: M and Z bands, N-lines and terminal cisternae of sarcoplasmic reticulum near the Z-bands.