Turnover of circulating hematopoietic stem cells
Dorie, M.J.; Maloney, M.A.; Patt, H.M.
Experimental Hematology 7(9): 483-489
1979
ISSN/ISBN: 0301-472X PMID: 527693 Document Number: 147343
Short-term parabiosis of male and female CBA/CaJ mice was used to investigate the turnover of circulating hematopoietic stem cells. The exchange and subsequent disappearance of donor stem cells were monitored by spleen colony assay and chromosome analysis of individual colonies. The results revealed an exponential disappearance of pluripotent stem cells from blood with a characteristic half time of 1.7 h. Blood-borne stem cells were shown to be equilibrated with a subpopulation of marrow stem cells exhibiting a disappearance half time of 9.5 h. Splenectomy did not change the apparent rate of stem cell removal from the blood.