Wild reservoirs and vectors of Trypanosoma cruzi. LXXII: Behavior of the PF strain of T. cruzi in mice inoculated with flagellates obtained from Triatominal infected by artificial xenodiagnosis or by inoculation into the coelomic cavity
Ribeiro, R.D.; Belda Neto, F.M.; Barretto, M.P.
Revista Brasileira de Biologia 39(1): 5-7
1979
ISSN/ISBN: 0034-7108 PMID: 111310 Document Number: 147189
Groups of 50 fifth-instar nymphs of Triatoma infestans and cultures of the PF strain of T. cruzi were used. Artificial xenodiagnosis infected 60.0% inoculation into the coelomic cavity infected 96.0%. In inoculations of the infected hemolymph into 20 clean white baby mice, an infection index of 20.0% was obtained; this percentage went up to 100% in subsequent reinoculations of mice with blood. Inoculations of feces from triatomines infected by artificial xenodiagnosis produced no positive results in mice. The inoculation of the PF strain into the coelomic cavity of bugs not only gave much better results than artificial xenodiagnoses, but also restored the virulence of the strain.