Use of the concanavalin-peroxidase-DAB system for identification of human chromosomes
Viguié, F.
Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales 173(4): 687-692
1979
ISSN/ISBN: 0037-9026 PMID: 160810 Document Number: 146488
The effect of concanavalin A on the prometaphase chromosomes was investigated, using a staining reaction based on the peroxydase-diaminobenzidin-H2O2 system. After incubation with concanavalina A, the chromosomes telomeres as well as the centromeres and satellites of the acrocentric chromosomes were strongly stained. Sometimes the chromatids appeared to be coiled. In other respect, it must be noted that peroxydase alone can stain the chromatids, which probably means that this compounds is able to unite with the chromosomes, without the aid of concanavalin A.