Use of isoenzyme and PCR markers for analyzing human chromosomes in human-rodent hybrid cells
Nestorova, T.B.; Kopantsev, E.P.; Borodin, A.M.; Zakiian, S.M.
Molekuliarnaia Biologiia 27(3): 685-707
1993
ISSN/ISBN: 0026-8984 PMID: 8316248 Document Number: 418501
The possibility of using isozyme and PCR markers for estimation of preservation of human chromosomes in human.rodent somatic cell hybrids is considered. Methods of electrophoretic separation of 33 isozymes and 11 PCR markers for 22 human autosomes and X chromosome are described. Using these isozymes and PCR primers as markers of known regions and arms of human chromosomes, one can avoid errors in typization of chromosomes with complicated rearrangements which simulate similar patterns of G-banding. The method proposed in the paper not only facilitates considerably the analysis of hybrid clones, but is also an easy and reliable tool in selection of somatic cell hybrid clones for further investigation.