Transformation of mouse lymphocytes stimulated by phytohemagglutin depending on their cultivation conditions
Meshcheriakova, I.E.; Surkova, E.A.; Poliak, R.I.
Tsitologiia 21(8): 982-985
1979
ISSN/ISBN: 0041-3771 PMID: 315124 Document Number: 145341
Conditions were elaborated to cultivate splenocytes of mice in medium-199 (Soviet production) changed a little by the addition of 200 mM of glutamine, 6 mM glucose, 60 unit/l of insulin, 5.10(-5) M 2-mercaptoethanol (final concentration), and of 5% serum of new-born calf. In such conditions of cultivation the value of the lymphocyte transformation coefficient lies within 3 and 10.
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