Somatic mutagenesis in human lymphocytes depending on genotypes for detoxification and oxidative response loci
Sal'nikova, L.E.; Chumachenko, A.G.; Akaeva, E.A.; Kuznetsova, G.I.; Vesnina, I.N.; Lapteva, N.S.; Abilev, S.K.; Rubanovich, A.V.
Genetika 46(12): 1678-1684
2010
ISSN/ISBN: 0016-6758 PMID: 21434421 Document Number: 647660
Associations of polymorphism of seven detoxification genes and three genes of oxidative response with the frequency of chromosome aberrations in human peripheral blood lymphocytes were studied. The genotyping data were correlated with the frequencies of spontaneous and gamma-induced (1 Gy in vitro) chromosome aberrations estimated for a group of healthy donors (97 males under 25 years of age) by analyzing 500-1000 metaphase cells per individual. The spontaneous level of aberrations of the chromosomal type was reduced in homozygotes for the GSTM1 locus deletion, and especially in double homozygotes for deletions of the GSTM1 and GSTT1 genes. The frequency of gamma-induced chromosome aberrations was reduced in G/G homozygotes for the minor allele of the poorly studied CYP1A1 T606G site: 0.094 +/- 0.006 against 0.112 +/- 0.002 for T allele carriers (P = 0.004). Linkage of the T606G site with well known and functionally important sites of the CYP1A1 gene (A4889G, T3801C) was analyzed.