Enzymatic assay for the antitumor agent-N- (phosphonacetyl) -L-aspartic acid (PALA)
Friedman, J.; Moore, E.C.; Hall, S.W.; Loo, T.L.
Cancer Treatment Reports 63(1): 85-88
1979
ISSN/ISBN: 0361-5960 PMID: 421235 Document Number: 143243
A method is described for the assay of N-(phosphonacetyl)-L-aspartic acid in plasma or urine, based on inhibition of partially purified aspartate carbamoyltransferase from rat liver. Concentrations of N-(phosphonacetyl)-L-aspartic acid as low as 0.1 microgram/ml can be detected. Plasma disappearance and urinary excretion curves from one patient are shown as examples of the application of the method.
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